For each sample, 1 g of total RNA was reverse-transcribed using a high-capacity cDNA reverse transcription kit (Applied Biosystems, Foster City, CA)
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Under regular circumstances, GH (growth hormone) binds to its receptor in the liver, and other tissues, and stimulates the synthesis/secretion of IGF-1. In target tissues, the Type 1 IGF receptor, that is homologous to the insulin receptor, is activated by IGF-1, leading to intracellular signaling which stimulates multiple processes leading to statural growth
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In rare cases, folate deficiency is associated with megaloblastic anemia and birth defects, especially neural tube defects, in children born to mothers with folate deficiency
First, neonate birth weight could be influenced by many internal and external factors, including sex hormones, diet, medicine, and heredity